Typicalquestions for the position of Analytical Chemist (1) 1. Whatis reverse phase? What is normal phase?
2. How to sample finished product?
3. What is the final point of titration?
4. For IR, what wavenumber is for peak of alcohol?
5. For GC, baseline is not stable? Why?
6. What are your weaknesses and strengths?
7. Introduce yourself?
8. How to prepare IR sample of liquid and solid?
9. What kind of instrument do you use?
10. What is std of KF?
11. Give an example of HPLC troubleshooting?
12. What are parameters of method validation? Whatkind of medicine did you test for method validation?
13. How many people are there in your QC department?
14. For assay, how many injections do you test forstd and sample?
15. How did you calibrate HPLC?
16. What are robustness and ruggedness?
17. What were your biggest accomplishments?
18. Do you prefer working with others or alone?
19. How do you get along with co-workers?
20. What difference is between FTIR and dispersive IR?
21 How to perform HPLC instrument validation?
Typicalquestions for the position of Analytical Chemist (2)
1. For GC, how do you solve that two peaks can’t be separated?
Change the column flow or oven program temperature,…to extend the retention time. If it still doesn’t work, change the column.
2. What factors affect retention time?
Stationary phase: type and properties, mobile phase: composition and properties, intermolecular forces between analyte & mobile phase, analyte & stationary phase, temperature. Flow…
3. What will you do if there is no peak, peak tailing and peak front?
Check the system. Any leak? Block in the injector? No sample in the vial? The inject volume too small, detector set point, or other incorrect set point.
4. What factors affect N?
Column performance: N=16(t/w)2, N=5.54(tr/w0.5)square. H=L/N.
loss of banded phase, column voiding, column contamination.
5. How do you perform tablet content uniformity?
6. What will you do if your experiment fail out?
Check stystem….check method,,,,check right sample…and procedure…OOS.
7. Why use buffer to adjust pH?
Buffer is used as standard to calibrate the pH meter in order that we can get the true pH value of samples. The pH value of buffer is always constant.
8. What will you do if the results are different for the same vail?
Calculate the RSD…if is less than 1%, it is ok. If RSD more than 1%, check the system ….column, setpoint, leak…
9. What is the reason that should have one peak but it have two peaks now?
Deintegration, degrade…contamination,..
10. Dissolution steps?
11. What is the theory of FID of GC ?
The FID burns the gas in a hydrogen flame. Organic compounds produce positive ions, which are collected at an electrode above the flame. The generated current is measured across the collector and the metal jet.
12. What kinds of detectors are used for HPLC?
UV detector or photodiode array, fluorescence, refractive index detector.
13. What will you do if RSD is not good?
14. What are included for SST//system suitability test?
relative retention time:a=(t2-t0)/(t1-t0), number of theoretical plates(N):N=16(t/w)square, no less than 500. capacity factor: k’=(t-t0)/t0; tailing factor: T=W0.05/2f, no more than 1.5. resolution between two peaks( R=2(t2-t1)/(w1+w2)). no less than 2.0%.
15. What is the cause of poor baseline?
Septum or Column bleed, column or detector Contamination, liner…carrier gas is not pure.
16. What will you do if Potency is 92.5%,but content uniformity are 95%,98%,102%(average is 101%)
17. What is USP accurate weight?
18. What is your weakness?
19. What will do if your opinions are different from you supervisor?
20. How do you deal with deadline?
21. What is the difference between normal phase and reverse phase?
药厂求职经验 我的求职历程
maplevoice.com 枫下之音
我的许多转折点都与14这个数字有关。14成了我的吉祥数字:2002年12月14日雅思考试并达到移民分数线;2003年3月14使馆来体检通知 书;2003年4月14日南京旅游顺便到南京红十字医院体检;2005年2月14日到加拿大第一个情人节接到公司的telephone interview通知,通知电话面试时间,名单。
2月17日下午1:00 电话面试:真巧,那两天电话铃声不知为何被设成静音。Dr. S 打电话来无人接听。后来改成打我手机。加上我的过分紧张,回答问题老停顿,第一印象不好。问的都是专业问题,针对我的简历问 HPLC,VALIDATION等方面问题。最后问我认识公司里什么人。交流不是非常顺畅,最后她说会put you on the schedule. 应该算是过了这一关。
2月22日:通知面试时间,名单。
3月7日9:00-12:00 到公司面试:先填applicationform and visitor card. 面试分四轮:
1) 三个Supervisor,其中两个没写到名单上,我名字也没记住。后边连thank youletter都没办法给她们寄。也许是刚开始,有点紧张,一见面竟然说:have a nice day! 这跟byebye没什么区别。她们没有事先准备好问题,我也没经验不知道怎么开始来个smalltalk. 场面很尴尬。闷约1分钟后他们陆续开始找到些问题并发问。气氛沉闷但还没出大差错。 其中有一个面试官是印度的,问的问题我老是听不太清楚。我连问了几个"Pardon me"把他问问题的欲望都给浇熄了。看他也不生气,始终一脸祥和,好人一个。
2) 两个Supervisor及一个manager assistant. 其中有个documentation 的supervisor苔蒂很nice. 一直在笑,有时不是很好笑的也笑得很大声,气氛马上缓和下来。我也开始轻松自若起来,当中OV对GC很在行,问我不少的问题,有点存心要把我考倒证明他更 厉害的味道。因而两个比较难的问题就抛给我了:a) 点火失败除了你刚才提到的原因还有什么原因。B) TCD不能用来测定哪些化合物?两个都没答上来,前边一个他告诉答案了,后一个就不了了之了。在交锋的过程中苔蒂一直在大笑,赞扬OV是在场唯一能把我考 倒的人,把气氛调了起来。这样也把我问题答不上的难堪扫去。没留下什么负面影响。当然有一两个问题也差点答错,是说KF的,我举例说在国内我用KF测定烟 丝中的的水份,,乔便问我烟丝能溶解在甲醇中吗? 我匆忙说我们公司是用KF测香精样品.香精是液态的,溶解没有问题.总的来说,这一轮人员更专业,问题更多。但经过这一轮我信心明显倍增。
3) Manager assistant(Dr.S) 和Manager:这轮是最关键的。Dr.S 看我镇定自若,一见面就说 Much better than Telephoneinterview。让我更添信心。Dr. S的问题还是老样。 回答起来很轻松。Manager的问题没有太专业问题,无非是强项,弱项等。
4) Manager of Human Resources: 这轮面试是太轻松了。因为决定权在用人部门,所以这轮没有压力。manager是一个干练的人,没有什么废话,问一通问题,比如电脑知识,同事相处等,唰 唰记好笔记就带我出门了。外边正下雨,问我怎么走,我说走路。她说会帮我叫一辆的士并cover费用。
打Taxi 到长途车站上了长途汽车,心里说不上什么感觉,好象有把握,好象又不是很有把握。有一点很欣慰的是总算对付过去了。第一次就能这样自我感觉也对得起两三个 星期的白天黑夜的努力。两天都没睡好,4小时每天。上车还是不想睡。有点失眠后的低度亢奋状态,头晕晕的。难怪面试的时候听力感觉下降了好多。让他们重复 了好几次问题。
正以为卸了一个大包袱,可以休整了,没想到两天后3月9日,人力资源部的经理打电话给我说还要同Director面试,因为3月7日那天他刚好没空。考虑 他周六将上Toronto,要我自己打电话给他联系见面时间地点。这可真是大难题啊。定好时间地点让我去参加面试我肯定成竹在胸,不会觉得有什么顾虑得。 可是让我打电话给面试名单里职务最高的Director联系面试时间地点等,真有点难为我的打电话技巧和听力。可是也没办法啊。就先练了练,打了近10次 到他办公室都没找到他,有点急了就留言,其实留言没有用,怎么好意思让他主动给我回电话呢?近中午时间终于联系上他了,报上名字他没印象----这当然。 于是赶紧说Setup time and location for Saturday’s interview. 他明白过来了。说对多伦多不熟悉,他想好地方给我发邮件,想了想又说你先给我发邮件吧。我记下邮件地址,让他重复了几遍,怕记不准确。 唉,可怜的听力和打电话技巧。放下电话我又想我去找地方吧,于是中午赶忙又去周末他要去办事的附近找coffee shop. 特意跑到WORKSHOP培训的地方问培训老师当地人喝咖啡喜欢什么咖啡店。说首先不能选coffee time 和Timhorrtons, 这些不是专业的咖啡馆。她们推荐了两家Second cup 和Timpthys。我把附近的咖啡店列了3家Email给Director, 他挑了我写的No.1Second cup. 并通过Email确定了那天会面时间:下午2点。我给他我的手机号,他也把他的号码给我了。接下来又是问WORKSHOP老师咖啡店面试有什么注意地方, 要不要给他点咖啡,自己喝什么,什么位置好等等。一切平稳下来,对面试也没有什么担忧的,毕竟这是最后一关了,证明前面的路都走正确了,于是非常的自信起 来。面试那天终于等来了,见面很严肃,问的全是专业问题(也难怪,他是博士啊),还掏出笔和纸来,划了一个液相色谱图,是周期性的基线噪音,问我什么原因 造成。我答Pump。他笑了笑,气氛缓和下来,然后我给他看我的GC,HPLC,GC/MS的培训证书和在德国的会议交流论文及我新近发表的一篇论文的英 文摘要。他看的非常的仔细,说了句well done. 最后同我聊了两三句中国公共场所能不能抽烟等。这比专业问题还不好答,呵呵。没什么词,随便对付答了几句。15分钟过去了,他起身同我握手,说了句 well done并祝我好运成功。那天回到家睡觉神经放松了,不管结果如何,考试结束了。那种压力释放的感觉真好。
随后是漫长的等候,希望一次一次被燃起又一次一次随西沉的日落而熄灭。还好没等太久,3月17日公司来电话问我REFERENCE情况并通知我开始查REFERENCES。真是开心啊。可是等了1天又1天还是没有消息。漫长的等待,整整2周过去了,终于3月31日一大早来电话告诉我 准备录用我,问我有没有兴趣。接着是等信--OFFER。4月4日发出的OFFER,4月5日FEDEX送信未达,其实我整个上午都在家等着。4月6日下 午还没到,我给公司人力部经理发信让她帮忙TRACK 信件。后来我自己TAXI到FEDEX 办公室取信。收到了OFFER一路上好开心。4月7日签好字发传真回去并寄信回公司。公司问我用中文名还是英文名,后来想想还是用中文名,反正读起来也很 简单。
4月18日正式上班。
下边是job posting for this position.
Analytical Chemists—Reference #200510
The successful candidates should possess a M.Sc. in Analytical Chemistry orB.Sc. in Chemistry with a minimum of 2 years experience in the pharmaceuticalindustry. The ideal candidates will have extensive experience withHPLC, GC, UV, IR and a thorough knowledge of method development and/or validationand/or quality control testing. Candidates should possess anaptitude for troubleshooting analytical instruments, as well as good writtenand verbal communication skills.
|